Phosphonate analogues of aminoacyl adenylates
نویسندگان
چکیده
منابع مشابه
Synthesis of Adenosine 5'- (Aminoalkyl phosphonates and phosphinates) Analogues of Aminoacyl Adenylates
Translation of the genetic code and protein synthesis from amino acids takes place in the ribosome’s where tRNAs act as key intermediates. Correct translation of genetic information into the specific amino acids is dependent on aminoacyl-tRNA ́s. The synthesis of these is catalyzed by aminoacyl-tRNA synthetase. The aminoacylation reaction, the so-called charging of tRNA is carried out in two ste...
متن کاملEditing of non-cognate aminoacyl adenylates by peptide synthetases.
Non-ribosomally formed peptides display both highly conserved and variable amino acid positions, the variations leading to a wide range of peptide families. Activation of the amino acid substrate proceeds in analogy to the ribosomal biosynthetic mechanism generating aminoacyl adenylate and acyl intermediates. To approach the mechanism of fidelity of amino acid selection, the stability of the am...
متن کاملEvidence for single mechanism for aminoacyl-tRNA synthetases including aminoacyl adenylates as intermediates.
The rate of transfer of amino acid from enzyme-bound aminoacyl adenylate to tRNA has been compared with the rate of esterification of free amino acid. The approach of Lövgren et al. (Lövgren, T. N. E., Heinonen, J., and Loftfield, R. B. (1975) J. Biol. Chem. 250, 3854-3860) was used, with 14C in the aminoacyl adenylate and 3H in the free amino acid and with both the lysine and isoleucine system...
متن کاملSynthesis of phosphonate and phostone analogues of ribose-1-phosphates
The synthesis of phosphonate analogues of ribose-1-phosphate and 5-fluoro-5-deoxyribose-1-phosphate is described. Preparations of both the alpha- and beta-phosphonate anomers are reported for the ribose and 5-fluoro-5-deoxyribose series and a synthesis of the corresponding cyclic phostones of each alpha-ribose is also reported. These compounds have been prepared as tools to probe the details of...
متن کاملSide Chain Independent Recognition of Aminoacyl Adenylates by the Hint1 Transcription Suppressor
Human Hint1 suppresses specific gene transcription by interacting with the transcription factor MITF in mast cells. Hint1 activity is connected to lysyl-tRNA synthetase (LysRS), a member of the universal aminoacyl tRNA synthetase family that catalyzes specific aminoacylation of their cognate tRNAs, through an aminoacyl adenylate (aa-AMP) intermediate. During immune activation, LysRS produces a ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1978
ISSN: 0264-6021
DOI: 10.1042/bj1750461